H⁺ + menaquinol-8 + fumarate → menaquinone-8 + succinate (R6531)
- Silencing
  - Genome
acetyl-CoA + phosphate ↔ coenzyme A + acetyl phosphate (R424)
- Silencing
  - Genome
NAD⁺ + (R)-lactate ← NADH + pyruvate + H⁺ (R504)
- Silencing
  - Genome
ethanol + NAD⁺ ↔ acetaldehyde + NADH + H⁺ (R157)
- Silencing
  - Genome
coenzyme A + acetaldehyde + NAD⁺ ↔ NADH + H⁺ + acetyl-CoA (R156)
- Silencing
  - Genome
H⁺ + (S)-2-acetolactate → (R)-acetoin + CO₂ (R532)
- Overexpression
  - Plasmid
pyruvate + H⁺ → (S)-2-acetolactate + CO₂ (R545)
- Overexpression
  - Plasmid
(R,R)-2,3-butanediol + NAD⁺ ↔ (R)-acetoin + NADH + H⁺ (R1993)
- Overexpression
  - Plasmid
S-adenosyl-L-methionine + demethylmenaquinol-8 → S-adenosyl-L-homocysteine + menaquinol-8 + H⁺ (R334)
- Attenuation
  - Genome
    - Escherichia coli UbiE117-BDO
menaquinol-8 + NAD⁺ ↔ menaquinone-8 + NADH + H⁺ (R00891)
- Silencing
  - Genome
    - Escherichia coli ETC1‐BDO
    - Escherichia coli ETC2‐BDO
NADH + ubiquinone-8 + H⁺ → ubiquinol-8 + NAD⁺ (R00892)
- Silencing
  - Genome
    - Escherichia coli ETC1‐BDO
    - Escherichia coli ETC2‐BDO
NADH + ubiquinone-8 + H⁺ → ubiquinol-8 + NAD⁺ + H⁺ (R6528)
- Silencing
  - Genome
    - Escherichia coli ETC3‐BDO
    - Escherichia coli ETC4‐BDO
menaquinol-8 + NAD⁺ + H⁺ ↔ menaquinone-8 + NADH + H⁺ (R00893)
- Silencing
  - Genome
    - Escherichia coli ETC3‐BDO
    - Escherichia coli ETC4‐BDO