• acetyl-CoA ↔ coenzyme A + acetoacetyl-CoA (R12)
  • Overexpression
    • Plasmid
      • Escherichia coli XL1-Blue/pMVA2
      • Escherichia coli NM522/pMVA2
• NADP⁺ + coenzyme A + (R)-mevalonate ↔ NADPH + H⁺ + (S)-3-hydroxy-3-methylglutaryl-CoA (R668)
  • Overexpression
    • Plasmid
      • Escherichia coli XL1-Blue/pMVA2
      • Escherichia coli NM522/pMVA2
• H₂O + acetoacetyl-CoA + acetyl-CoA ↔ coenzyme A + H⁺ + (S)-3-hydroxy-3-methylglutaryl-CoA (R685)
  • Overexpression
    • Plasmid
      • Escherichia coli XL1-Blue/pMVA2
      • Escherichia coli NM522/pMVA2