phosphoenolpyruvate + β-D-glucopyranose → β-D-glucose 6-phosphate + pyruvate (R4216)
- Silencing
  - Genome
H₂O + pyruvate + ubiquinone-8 → acetate + ubiquinol-8 + CO₂ (R4535)
- Silencing
  - Genome
D-Glucose + H⁺ → D-Glucose + H⁺ (R00800)
- Overexpression
  - Genome
NAD⁺ + (R)-lactate ← NADH + pyruvate + H⁺ (R504)
- Silencing
  - Genome
H⁺ + menaquinol-8 + fumarate → menaquinone-8 + succinate (R6531)
- Silencing
  - Genome
butanoyl-CoA + NAD⁺ ↔ crotonyl-CoA + NADH + H⁺ (R2751)
- Overexpression
  - Genome
  - Plasmid
    - Escherichia coli JHL26
(S)-3-hydroxybutanoyl-CoA ↔ H₂O + crotonyl-CoA (R737)
- Overexpression
  - Genome
  - Plasmid
    - Escherichia coli JHL26
- Silencing
  - Genome
    - Escherichia coli JHL26
ethanol + NAD⁺ ↔ acetaldehyde + NADH + H⁺ (R157)
- Silencing
  - Genome
coenzyme A + acetaldehyde + NAD⁺ ↔ NADH + H⁺ + acetyl-CoA (R156)
- Silencing
  - Genome
acetyl-CoA ↔ coenzyme A + acetoacetyl-CoA (R12)
- Overexpression
  - Genome
(S)-3-hydroxybutanoyl-CoA + NAD⁺ ↔ NADH + acetoacetyl-CoA + H⁺ (R166)
- Overexpression
  - Genome
  - Plasmid
    - Escherichia coli JHL26
acetate + butanoyl-CoA ↔ butanoate + acetyl-CoA (R718)
- Overexpression
  - Genome
    - Escherichia coli BuT-8LA
    - Escherichia coli BuT-8L-ato
acetyl-CoA + phosphate ↔ coenzyme A + acetyl phosphate (R424)
- Silencing
  - Genome
    - Escherichia coli BuT-8L-ato
    - Escherichia coli JHL26
acetyl-CoA + acetoacetate ↔ acetate + acetoacetyl-CoA (R745)
- Silencing
  - Genome
    - Escherichia coli JHL26
(S)-3-hydroxybutanoyl-CoA + NADP⁺ ← NADPH + acetoacetyl-CoA + H⁺ (R1390)
- Silencing
  - Genome
    - Escherichia coli JHL26
H2O + butanoyl-CoA → coenzyme A + H+ + butanoate (R00654)
- Overexpression
  - Plasmid
    - Escherichia coli JHL26