• coenzyme A + 4-coumarate + ATP → (E)-4-coumaroyl-CoA + diphosphate + AMP (R2776)
  • Overexpression
    • Plasmid
      • Escherichia coli B-AP4
      • Escherichia coli B-AP5
      • Escherichia coli B-AP6
      • Escherichia coli B-GK
• H⁺ + malonyl-CoA + (E)-4-coumaroyl-CoA → CO₂ + coenzyme A + naringenin chalcone (R8128)
  • Overexpression
    • Plasmid
      • Escherichia coli B-AP4
      • Escherichia coli B-AP5
      • Escherichia coli B-AP6
      • Escherichia coli B-GK
• naringenin chalcone → (2S)-naringenin (R11742)
  • Overexpression
    • Plasmid
      • Escherichia coli B-AP4
      • Escherichia coli B-AP5
      • Escherichia coli B-AP6
      • Escherichia coli B-GK
• (2S)-naringenin + 2-oxoglutarate + oxygen → H₂O + H⁺ + succinate + CO₂ + apigenin (R10337)
  • Overexpression
    • Plasmid
      • Escherichia coli B-AP4
      • Escherichia coli B-AP5
      • Escherichia coli B-AP6
      • Escherichia coli B-GK
• L-tyrosine → 4-coumarate + ammonium (R3408)
  • Overexpression
    • Plasmid
      • Escherichia coli B-AP4
      • Escherichia coli B-AP5
      • Escherichia coli B-AP6
      • Escherichia coli B-GK
• D-erythrose 4-phosphate + phosphoenolpyruvate + H₂O ↔ 3-deoxy-D-arabino-heptulosonate 7-phosphate + phosphate (R576)
  • Feedback insensitive
    • Plasmid
      • Escherichia coli B-AP6
      • Escherichia coli B-GK
  • Overexpression
    • Plasmid
      • Escherichia coli B-AP5
• prephenate + NAD⁺ → NADH + 3-(4-hydroxyphenyl)pyruvate + CO₂ (R727)
  • Feedback insensitive
    • Plasmid
      • Escherichia coli B-AP6
      • Escherichia coli B-GK
  • Overexpression
    • Plasmid
      • Escherichia coli B-AP5
• chorismate ↔ prephenate (R69)
  • Feedback insensitive
    • Plasmid
      • Escherichia coli B-AP6
      • Escherichia coli B-GK
  • Overexpression
    • Plasmid
      • Escherichia coli B-AP5
• S-adenosyl-L-methionine + apigenin → S-adenosyl-L-homocysteine + genkwanin (R9735)
  • Overexpression
    • Plasmid
      • Escherichia coli B-GK