acetyl-CoA + L-serine ↔ coenzyme A + O-acetyl-L-serine (R133)
- Feedback insensitive
  - Plasmid
  - Genome
- Overexpression
  - Plasmid
    - Escherichia coli BW02/pTrc99a-cysE
L-cysteine → L-cysteine (R00799)
- Overexpression
  - Plasmid
H₂O + L-cysteine → pyruvate + ammonium + hydrogen sulfide (R2399)
- Silencing
  - Genome
L-serine → pyruvate + ammonium (R1112)
- Silencing
  - Genome
NAD⁺ + 3-phospho-D-glycerate ↔ 3-phosphooxypyruvate + NADH + H⁺ (R51)
- Feedback insensitive
  - Plasmid
  - Genome
- Overexpression
  - Genome
H₂O + 3-phospho-L-serine → L-serine + phosphate (R429)
- Overexpression
  - Plasmid
  - Genome
2-oxoglutarate + 3-phospho-L-serine ↔ 3-phosphooxypyruvate + L-glutamate (R555)
- Overexpression
  - Plasmid
  - Genome
O-acetyl-L-serine + hydrogen sulfide ↔ acetate + H⁺ + L-cysteine (R684)
- Overexpression
  - Genome
  - Plasmid
    - Escherichia coli W3110 pCysK
    - Escherichia coli W3110 pCysM
hydrogen cyanide + thiosulfate → H⁺ + thiocyanate + sulfite (R1121)
- Overexpression
  - Genome
  - Plasmid
    - Escherichia coli ΔcysM/pDES/pGlpE
    - Escherichia coli WT/pDES/pGlpE
H₂O + glycine + 5,10-methylene-tetrahydromethanopterin ↔ tetrahydromethanopterin + L-serine (R10280)
- Attenuation
  - Genome
- Silencing
  - Genome
    - Escherichia coli BW08∆glyA/pE
β-D-fructose 1,6-bisphosphate ↔ glycerone phosphate + D-glyceraldehyde 3-phosphate (R365)
- Overexpression
  - Genome
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate ↔ D-erythrose 4-phosphate + β-D-fructofuranose 6-phosphate (R492)
- Overexpression
  - Genome
D-glyceraldehyde 3-phosphate ↔ glycerone phosphate (R232)
- Overexpression
  - Genome
    - Escherichia coli BW13-1/pE
    - Escherichia coli BW13-3/pE
β-D-glucose 6-phosphate + NADP⁺ → 6-phospho D-glucono-1,5-lactone + NADPH + H⁺ (R392)
- Overexpression
  - Genome
    - Escherichia coli BW14-1/pE