hercynine + oxygen + γ-L-glutamyl-L-cysteine → H₂O + γ-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide (R10592)
- Overexpression
  - Plasmid
    - Escherichia coli ET3
    - Escherichia coli ET4
H₂O + γ-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide → L-glutamate + S-(hercyn-2-yl)-L-cysteine S-oxide (R6992)
- Overexpression
  - Plasmid
    - Escherichia coli ET3
    - Escherichia coli ET4
a reduced electron donor + S-(hercyn-2-yl)-L-cysteine S-oxide → an oxidized electron donor + pyruvate + ammonium + ergothioneine (R10940)
- Overexpression
  - Plasmid
    - Escherichia coli ET3
    - Escherichia coli ET4
S-adenosyl-L-methionine + L-histidine → S-adenosyl-L-homocysteine + H⁺ + hercynine (R4089)
- Overexpression
  - Plasmid
    - Escherichia coli ET3
    - Escherichia coli ET4
L-glutamate + L-cysteine + ATP → H⁺ + phosphate + ADP + γ-L-glutamyl-L-cysteine (R32)
- Overexpression
  - Plasmid
    - Escherichia coli ET4