• D-erythrose 4-phosphate + phosphoenolpyruvate + H₂O ↔ 3-deoxy-D-arabino-heptulosonate 7-phosphate + phosphate (R576)
  • Feedback insensitive
    • Plasmid
      • Escherichia coli DG01
      • Escherichia coli DG02
      • Escherichia coli DG03
• prephenate + H⁺ ↔ H₂O + 3-phenyl-2-oxopropanoate + CO₂ (R511)
  • Feedback insensitive
    • Plasmid
      • Escherichia coli DG01
      • Escherichia coli DG02
      • Escherichia coli DG03
• chorismate ↔ prephenate (R69)
  • Feedback insensitive
    • Plasmid
      • Escherichia coli DG01
      • Escherichia coli DG02
      • Escherichia coli DG03
• 3-phenyl-2-oxopropanoate + H⁺ → phenylacetaldehyde + CO₂ (R2072)
  • Overexpression
    • Plasmid
      • Escherichia coli DG01
      • Escherichia coli DG02
      • Escherichia coli DG03
• H₂O + phenylacetaldehyde + NAD⁺ ↔ H⁺ + phenylacetate + NADH (R2034)
  • Overexpression
    • Plasmid
      • Escherichia coli DG01
      • Escherichia coli DG02
      • Escherichia coli DG03
• 3-phenyl-2-oxopropanoate + L-glutamate ↔ L-phenylalanine + 2-oxoglutarate (R363)
  • Overexpression
    • Plasmid
      • Escherichia coli DG02
      • Escherichia coli DG03
• 2-phenylethanol + acetyl-CoA → coenzyme A + phenylethyl acetate (R6592)
  • Overexpression
    • Plasmid
      • Escherichia coli DG03