• (2E,6E)-farnesyl diphosphate → diphosphate + valerena-4,7(11)-diene (R9379)
  • Overexpression
    • Plasmid
      • Escherichia coli DH5αZ1 (pZE-wvds)
      • Escherichia coli DH5αZ1 (pZE-wvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pZE-cvds)
      • Escherichia coli DH5αZ1 (pZE-cvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-wvds)
      • Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-cvds)
      • Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• pyruvate + H⁺ + D-glyceraldehyde 3-phosphate → 1-deoxy-D-xylulose 5-phosphate + CO₂ (R270)
  • Overexpression
    • Plasmid
      • Escherichia coli DH5αZ1 (pZE-cvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pZE-wvds)/(pSTV-dxs-idi-fps)
• isopentenyl diphosphate ↔ prenyl diphosphate (R123)
  • Overexpression
    • Plasmid
      • Escherichia coli DH5αZ1 (pZE-wvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pZE-cvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• isopentenyl diphosphate + prenyl diphosphate ↔ diphosphate + geranyl diphosphate (R315)
  • Overexpression
    • Plasmid
      • Escherichia coli DH5αZ1 (pZE-wvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pZE-cvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• isopentenyl diphosphate + geranyl diphosphate ↔ diphosphate + (2E,6E)-farnesyl diphosphate (R574)
  • Overexpression
    • Plasmid
      • Escherichia coli DH5αZ1 (pZE-wvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pZE-cvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/(pSTV-dxs-idi-fps)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• acetyl-CoA ↔ coenzyme A + acetoacetyl-CoA (R12)
  • Overexpression
    • Plasmid
      • Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• H₂O + acetoacetyl-CoA + acetyl-CoA ↔ coenzyme A + H⁺ + (S)-3-hydroxy-3-methylglutaryl-CoA (R685)
  • Overexpression
    • Plasmid
      • Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• NADP⁺ + coenzyme A + (R)-mevalonate ↔ NADPH + H⁺ + (S)-3-hydroxy-3-methylglutaryl-CoA (R668)
  • Overexpression
    • Plasmid
      • Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• (R)-mevalonate + ATP ↔ (R)-5-phosphomevalonate + H⁺ + ADP (R630)
  • Overexpression
    • Plasmid
      • Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• (R)-5-phosphomevalonate + ATP ↔ (R)-mevalonate diphosphate + ADP (R716)
  • Overexpression
    • Plasmid
      • Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• (R)-mevalonate diphosphate + ATP → isopentenyl diphosphate + phosphate + ADP + CO₂ (R465)
  • Overexpression
    • Plasmid
      • Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
      • Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)