Name	Genotype
Escherichia coli 7101-17 (DE3)	Δnag::tetR manXYZ8 DE3
Escherichia coli 2123-12	ΔlacZ::T7lac–glmS–CamR
Escherichia coli 2123-54	ΔlacZ::T7lac–glmS*54–CamR
Escherichia coli 7107-18	ΔgalK::T7lac–glmS*54
Escherichia coli 7107-88
Escherichia coli 7107-87
Escherichia coli 7107-93
Escherichia coli 7107-117
Escherichia coli 7107-92	ΔmanXYZ::T7lac-ScGNA1
Escherichia coli 7107-607	ΔfucIK::T7lac–ScGNA1
Escherichia coli 7107-608	ΔtreB::T7lac–ScGNA1
Escherichia coli 7107-609	ΔmelAB::T7lac–ScGNA1
Escherichia coli 7107-612	ΔmelAB::T7lac–ScGNA1
Escherichia coli Y4-1	mbhA::Ptrc-ushA, gapC::Ptrc-nagD, arpB::Ptrc-surE
Escherichia coli Y4-2	ylbe::Ptrc-isn1Sc
Escherichia coli Y4-3	∆hpt
Escherichia coli Y5-1	∆guaB
Escherichia coli Y5-2	rph::PapFAB104-EsaR
Escherichia coli Y5-3	yghe::PesaS-guaB
Escherichia coli Y5-4A	ycdn:: PBBa_j 23114esaI
Escherichia coli Y5-4B	ycdn:: PBBa_j 23110esaI
Escherichia coli Y5-4C	ycdn:: PBBa_j 23101esaI
Escherichia coli Y5-4D	ycdn:: PBBa_j 23100esaI
Escherichia coli Y6	yjip::Ptrc-pur(FDNTSQLMKECB)
Escherichia coli Y6-1	yeel::Ptrc- glnABsL159I, E304A
Escherichia coli Y6-2	yjgx::Plac-aspC
Escherichia coli Y6-3	∆thrA
Escherichia coli Y6-4	ynci:: PBBa_j 23109-thrA
Escherichia coli Y6-5	ynci:: PBBa_j 23114-thrA
Escherichia coli Y6-6	ynci:: PBBa_j 23105-thrA
Escherichia coli Y6-7	ynci:: PBBa_j 23110-thrA
Escherichia coli Arg3	yghX::PBJ23119-B0034-argCBHEc, yncI::PBJ23119-B0034-argA*, rpnD::PBJ23119-B0034-argD, ycjV::PBJ23119-B0034-argI and yrfG::PBJ23119-B0034-argG
Escherichia coli Arg4	artJ::PBJ23119-B0034-lysECg and argT::PBJ23119-B0034-cgmACg
Escherichia coli Arg5	yacC::PBJ23119-B0034-gdhASt and ydjK::PBJ23119-B0034-gdhASt
Escherichia coli Arg6	Picd:PBJ23100-B0034
Escherichia coli Arg7	Pppc:PBJ23114-B0034
Escherichia coli Arg8	PBJ23110-B0034-aspC and PBJ23115-B0034-gltA
Escherichia coli Arg9	yafF::PBJ23119-B0034-glnABs
Escherichia coli Arg10	PBJ23110-B0034-pyrF and PBJ23118-B0034-argF
Escherichia coli Arg4-1
Escherichia coli Arg4-2
Escherichia coli Arg4-3
Escherichia coli Arg4-4
Escherichia coli Arg4-5
Escherichia coli Arg4-6
Escherichia coli Arg4-7	CRISPRi-downregulated pyrF
Escherichia coli Arg4-8	CRISPRi-downregulated gltA
Escherichia coli Arg4-9
Escherichia coli Arg4-10
Escherichia coli Arg4-11
Escherichia coli Arg4-12
Escherichia coli Arg4-13
Escherichia coli Arg4-14
Escherichia coli Arg4-15
Escherichia coli Arg4-16
Escherichia coli Arg4-17
Escherichia coli Arg4-18
Escherichia coli Arg4-19
Escherichia coli Arg5-1	PBJ23100-B0034-icd
Escherichia coli Arg5-2	PBJ23104-B0034-icd
Escherichia coli Arg5-3	PBJ23118-B0034-icd
Escherichia coli Arg6-1	PBJ23110-B0034-ppc
Escherichia coli Arg6-2	PBJ23114-B0034-ppc
Escherichia coli Arg6-3	PBJ23115-B0034-ppc
Escherichia coli Arg6-4	PBJ23118-B0034-ppc
Escherichia coli Arg6-5	PBJ23104-B0034-ppc
Escherichia coli Arg6-6	PBJ23100-B0034-ppc
Escherichia coli Arg7-1	PBJ23100-B0034-aspC and PBJ23115-B0034-gltA
Escherichia coli Arg7-2	PBJ23104-B0034-aspC and PBJ23115-B0034-gltA
Escherichia coli Arg7-4	PBJ23100-B0034-aspC and PBJ23114-B0034-gltA
Escherichia coli Arg7-5	PBJ23104-B0034-aspC and PBJ23114-B0034-gltA
Escherichia coli Arg7-6	PBJ23110-B0034-aspC and PBJ23114-B0034-gltA
Escherichia coli Arg7-7	PBJ23100-B0034-aspC and PBJ23109-B0034-gltA
Escherichia coli Arg7-8	PBJ23104-B0034-aspC and PBJ23109-B0034-gltA
Escherichia coli Arg7-9	PBJ23110-B0034-aspC and PBJ23109-B0034-gltA
Escherichia coli Arg9-1	PBJ23110-B0034-pyrF and PBJ23110-B0034-argF
Escherichia coli Arg9-2	PBJ23110-B0034-pyrF and PBJ23115-B0034-argF
Escherichia coli Arg9-4	PBJ23115-B0034-pyrF and PBJ23110-B0034-argF
Escherichia coli Arg9-5	PBJ23115-B0034-pyrF and PBJ23115-B0034-argF
Escherichia coli Arg9-6	PBJ23115-B0034-pyrF and PBJ23118-B0034-argF
Escherichia coli HIS3-3	ΔmazG, Δamn, ΔygdH, ΔyrfG, ΔyjjG, ΔsurE, ΔushA
Escherichia coli HIS3-4	yncI::Ptrc-cipB-hisHAFI, rph::Ptrc-cipB-EcpurH
Escherichia coli HIS3-5	yghX::Ptrc-hisDCB
Escherichia coli HIS3-6	purR::Ptrc-cipA-Smprs, yjhE::Ptrc-cipA-tktA
Escherichia coli HIS3-7	yghE::Ptrc-cipA-Smprs
Escherichia coli HIS3-8	yeeP::Ptrc-zwf
Escherichia coli HIS3-9	ybeM::Ptrc-gnd
Escherichia coli HIS3-10	yjiP::Ptrc-BspurA
Escherichia coli HIS3-11	yjgX::Ptrc-BspurB
Escherichia coli HIS3-12	mbhA::Ptrc-Ecndk
Escherichia coli HIS3-13	ychG::Ptrc-Bsadk
Escherichia coli HIS4-1	yeeL::Ptrc-gdhA
Escherichia coli HIS4-2	yjiV::Ptrc-glnA
Escherichia coli HIS4-3	yjiV::PglnA-glnA
Escherichia coli HIS5-1	gapC::PsoxS-vgb
Escherichia coli HIS5-2	gapC::Pbfd-vgb
Escherichia coli HIS5-3	gapC::PexbB-vgb
Escherichia coli HIS5-4	gapC::PlgoR-vgb
Escherichia coli HIS5-5	Pcyo::PsoxS
Escherichia coli HIS5-6	Pcyo::Pbfd
Escherichia coli HIS5-7	Pcyo::PexbB
Escherichia coli HIS5-8	Pcyo::PlgoR
Escherichia coli HIS5-9	Pnuo::PsoxS
Escherichia coli HIS5-10	Pnuo::Pbfd
Escherichia coli HIS5-11	Pnuo::PexbB
Escherichia coli HIS5-12	Pnuo::PlgoR
Escherichia coli GIMP10	△ushA, △nagD
Escherichia coli GIMP11	△yrfG
Escherichia coli GIMP12	△surE
Escherichia coli AKN11	ΔnagD
Escherichia coli AKN12	ΔushA
Escherichia coli AKN13	△guaB
Escherichia coli AKN14	ΔpurA
Escherichia coli AKN15	PAICS::yncK
Escherichia coli AKN16	HPRT::ydeU
Escherichia coli AKN17	Ptac-glyA
Escherichia coli AKN18	Ptac-folD
Escherichia coli AKN19	Ptac-purH
Escherichia coli P1	ΔyrfG
Escherichia coli P2	ΔnagD
Escherichia coli P3	ΔushA
Escherichia coli P4	ΔsurE
Escherichia coli P9	ΔyrfG ΔushA
Escherichia coli P10	ΔushA ΔsurE
Escherichia coli P11	ΔnagD ΔushA
Escherichia coli P12	ΔyrfG ΔnagD ΔushA
Escherichia coli P13	ΔyrfG ΔnagD ΔushA ΔsurE
Escherichia coli BL21(DE3) ΔyrfG	ΔyrfG
Escherichia coli BL21(DE3) ΔnagD	ΔnagD
Escherichia coli BL21(DE3) ΔushA	ΔushA
Escherichia coli BL21(DE3) ΔsurE	ΔsurE
Escherichia coli HW4-1	△mazG, △ushA, △surE, △yjjG, △yrfG, △amn, △ygdH
Escherichia coli HW4-2	rph::Ptrc-purH
Escherichia coli HW4-3	ycjV::Ptrc-purA
Escherichia coli HW4-4	yjiP::Ptrc-purB
Escherichia coli HW4-5	△purF
Escherichia coli HW5-1	yjhE::Ptrc-rocG
Escherichia coli HW6-1	△hisJ
Escherichia coli HW6-2	△aroP
Escherichia coli HW6-3	yjiT::Ptrc-lysECg
Escherichia coli HW6-4	yjiT::Ptrc-argO