Escherichia coli S028

Ancestors

Derived strains

Genotype with respect to parental

P_trc -trpE^fbrDCBA

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacU169 gal490 λcI857 Δ(cro-bioA) | rpsL (StrR) | ∆aroF ∆aroG ∆mtr ∆tnaA ∆tnaB ∆λ | ∆aroH::P_{J23119-rpsL-tac} -(aroG^fbr-serA^fbr) | P_trc -trpE^fbrDCBA

Bars (|) indicate differences between strains.

Modifications

Origin	Gene	Organism	Modification
Escherichia coli S015	aroF	Escherichia coli	Silencing
Escherichia coli S015	aroG	Escherichia coli	Silencing
Escherichia coli S015	mtr	Escherichia coli	Silencing
Escherichia coli S015	tnaA	Escherichia coli	Silencing
Escherichia coli S015	tnaB	Escherichia coli	Silencing
Escherichia coli S019	aroH	Escherichia coli	Silencing
Escherichia coli S019	aroG	Escherichia coli	Feedback insensitive
Escherichia coli S019	serA	Escherichia coli	Feedback insensitive
Escherichia coli S028	trpE	Escherichia coli	Feedback insensitive
Escherichia coli S028	trpD	Escherichia coli	Overexpression
Escherichia coli S028	trpC	Escherichia coli	Overexpression
Escherichia coli S028	trpB	Escherichia coli	Overexpression
Escherichia coli S028	trpA	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan	Titer	19.0 g/L		Glucose	42 h	Fed-Batch	[165]
L-tryptophan	Substrate yield	0.15 g/g of sustrate		Glucose	42 h	Fed-Batch	[165]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Lin Chen & An-Ping Zeng (2016). Rational design and metabolic analysis of Escherichia coli for effective production of L-tryptophan at high concentration. Applied Microbiology and Biotechnology.

Lin Chen, Minliang Chen, Chengwei Ma & An-Ping Zeng (2018). Discovery of feed-forward regulation in L-tryptophan biosynthesis and its use in metabolic engineering of E. coli for efficient tryptophan bioproduction. Metabolic Engineering.

José-Aníbal Mora-Villalobos & An-Ping Zeng (2018). Synthetic pathways and processes for effective production of 5-hydroxytryptophan and serotonin from glucose in Escherichia coli. Journal of Biological Engineering.