Escherichia coli ALA24

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pZPW86 added | Plasmid pZCA136 added

Bars (|) indicate differences between strains.

pZCA136 (sRsucC, sRhemB, sRaceA, P_tet, T1/TE)

pZPW86 (hemA^C75A/R365K, P_J23100-coaA, P_ppc-ppc, P_eamA-eamA, P_grxA-grxA, P_sodA-sodA)

Origin	Gene	Organism	Modification
pZCA136	sucC	Escherichia coli	Attenuation
pZCA136	hemB	Escherichia coli	Attenuation
pZCA136	aceA	Escherichia coli	Attenuation
pZPW86	hemA	Rhodopseudomonas palustris	Feedback insensitive
pZPW86	coaA	Escherichia coli	Overexpression
pZPW86	ppc	Escherichia coli	Overexpression
pZPW86	eamA	Escherichia coli	Overexpression
pZPW86	grxA	Escherichia coli	Overexpression
pZPW86	sodA	Escherichia coli	Overexpression

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
5-aminolevulinate	Titer	8.539 g/L ^*		Glucose		Flask	[239]
5-aminolevulinate	Titer	8.539 g/L ^*	6.662 OD₆₀₀^*	Glucose		Flask	[239]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.