Escherichia coli B3/pED
Ancestors
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | ΔtrpR | ΔpheA | Plasmid pED addedBars (|) indicate differences between strains.
Plasmids
-
pED (PT7-trpEfbr (Met293Thr, TCC to TTC; Ser40Leu ATG to ACG)-trpD)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Feedback insensitive
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Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tryptophan | Titer | 0.1115 g/L * | 3.857 OD600* | 48 h | Flask | [96] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.