Escherichia coli B4/pED + pVio

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | ΔtnaA | Plasmid pVio added | Plasmid pED added

Bars (|) indicate differences between strains.

pED (P_T7-trpE^fbr (Met293Thr, TCC to TTC; Ser40Leu ATG to ACG)-trpD)

pVio (vioABCDE)

Origin	Gene	Organism	Modification
Escherichia coli B4	tnaA	Escherichia coli	Silencing
pED	trpE	Escherichia coli	Feedback insensitive
pED	trpD	Escherichia coli	Overexpression
pVio	vioA	Chromobacterium violaceum	Overexpression
pVio	vioB	Chromobacterium violaceum	Overexpression
pVio	vioC	Chromobacterium violaceum	Overexpression
pVio	vioD	Chromobacterium violaceum	Overexpression
pVio	vioE	Chromobacterium violaceum	Overexpression

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
violacein	Titer	0.5824 g/L ^*	2.221 OD₆₀₀^*	Glucose	48 h	Flask	[96]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.