Escherichia coli D (pS5, pY1)
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pS5 added | Plasmid pY1 addedBars (|) indicate differences between strains.
Plasmids
-
pS5 (pBBR1 ori, lacI, Plac-UV5-aroE-aroD-aroBop-T1-Ptrc-aroG*-ppsA-tktA)
pY1 (p15A ori, Plac-UV5-tyrB-tyrA*-aroC-aroA-aroL)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Overexpression
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Overexpression
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Overexpression
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tyrosine | Titer | 1219.0 mg/L | 24 h | Flask | [244] | ||
| L-tyrosine | Biomass-specific yield | 1451.0 mg/g DCW | 24 h | Flask | [244] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.