Escherichia coli F (pS4, pY3)
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pS4 added | Plasmid pY3 addedBars (|) indicate differences between strains.
Plasmids
-
pS4 (pBBR1 ori, aroE-aroD-aroBop-PLtetO-1-aroG*-ppsA-tktA)
pY3 (p15A ori, lacI,Plac-UV5-tyrB-tyrA*-aroC T1-Ptrc-aroA-aroL)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Overexpression
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Overexpression
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Overexpression
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tyrosine | Titer | 2169.0 mg/L | 48 h | Flask | [244] | ||
| L-tyrosine | Biomass-specific yield | 2645.0 mg/g DCW | 48 h | Flask | [244] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.