Escherichia coli RF03T

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli RF02

Escherichia coli RF03

Escherichia coli RF03T

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Δpgi | Δedd, Δeda | Plasmid pLS01 added

Bars (|) indicate differences between strains.

Plasmids

pLS01 (pSC101 replicon, P_trc ΔlacI^q ΔlacO-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)

Modifications

Origin	Gene	Organism	Modification
Escherichia coli RF02	pgi	Escherichia coli	Silencing
Escherichia coli RF03	edd	Escherichia coli	Silencing
Escherichia coli RF03	eda	Escherichia coli	Silencing
pLS01	ribA	Escherichia coli	Overexpression
pLS01	ribB	Escherichia coli	Overexpression
pLS01	ribD	Escherichia coli	Overexpression
pLS01	ribE	Escherichia coli	Overexpression
pLS01	ribC	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
riboflavin	Titer	516.0 mg/L		Glucose	23 h	Flask	[247]
riboflavin	Titer	502.0 mg/L	4.0 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	50.2 mg/g of sustrate	4.0 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	5.5 mg/g DCW/h	4.0 g/L	Glucose		Flask	[247]
riboflavin	Titer	540.0 mg/L	4.1 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	54.1 mg/g of sustrate	4.1 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	5.7 mg/g DCW/h	4.1 g/L	Glucose		Flask	[247]
riboflavin	Titer	570.0 mg/L	4.2 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	57.2 mg/g of sustrate	4.2 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	5.9 mg/g DCW/h	4.2 g/L	Glucose		Flask	[247]
riboflavin	Titer	597.0 mg/L	4.5 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	59.6 mg/g of sustrate	4.5 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	5.8 mg/g DCW/h	4.5 g/L	Glucose		Flask	[247]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Liu, Shuang; Kang, Pei; Cui, Zhenzhen; Wang, Zhiwen & Chen, Tao. Increased riboflavin production by knockout of 6-phosphofructokinase I and blocking the Entner–Doudoroff pathway in Escherichia coli. Biotechnology Letters. 2016, 38(8), 1307-1314.