Escherichia coli LS02T

Ancestors

Derived strains

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔpfkA, Δedd, Δeda | Plasmid pLS01 added

Bars (|) indicate differences between strains.

Plasmids

pLS01 (pSC101 replicon, P_trc ΔlacI^q ΔlacO-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)

Modifications

Origin	Gene	Organism	Modification
Escherichia coli LS02	pfkA	Escherichia coli	Silencing
Escherichia coli LS02	edd	Escherichia coli	Silencing
Escherichia coli LS02	eda	Escherichia coli	Silencing
pLS01	ribA	Escherichia coli	Overexpression
pLS01	ribB	Escherichia coli	Overexpression
pLS01	ribD	Escherichia coli	Overexpression
pLS01	ribE	Escherichia coli	Overexpression
pLS01	ribC	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
riboflavin	Titer	667.0 mg/L	3.8 g/L	Glucose		Flask	[247]
riboflavin	Titer	10.4 g/L	32.0 g/L	Glucose	71 h	Fed-Batch	[247]
riboflavin	Substrate yield	56.8 mg/g of sustrate	32.0 g/L	Glucose	71 h	Fed-Batch	[247]
riboflavin	Titer	5.8 g/L		Glucose		Fed-Batch	[247]
riboflavin	Substrate yield	67.9 mg/g of sustrate	3.8 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	7.8 mg/g DCW/h	3.8 g/L	Glucose		Flask	[247]
riboflavin	Titer	605.0 mg/L	3.9 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	61.7 mg/g of sustrate	3.9 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	6.7 mg/g DCW/h	3.9 g/L	Glucose		Flask	[247]
riboflavin	Titer	551.0 mg/L	3.9 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	53.4 mg/g of sustrate	3.9 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	6.1 mg/g DCW/h	3.9 g/L	Glucose		Flask	[247]
riboflavin	Titer	327.0 mg/L	4.2 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	31.8 mg/g of sustrate	4.2 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	3.4 mg/g DCW/h	4.2 g/L	Glucose		Flask	[247]
riboflavin	Titer	296.0 mg/L	4.4 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	26.8 mg/g of sustrate	4.4 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	2.9 mg/g DCW/h	4.4 g/L	Glucose		Flask	[247]
riboflavin	Titer	605.0 mg/L	3.2 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	59.9 mg/g of sustrate	3.2 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	9.1 mg/g DCW/h	3.2 g/L	Glucose		Flask	[247]
riboflavin	Titer	44.7 mg/L	2.7 g/L	Glucose		Flask	[247]
riboflavin	Substrate yield	4.9 mg/g of sustrate	2.7 g/L	Glucose		Flask	[247]
riboflavin	Biomass-specific productivity	0.4 mg/g DCW/h	2.7 g/L	Glucose		Flask	[247]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Dai, Jiu; Geng, Mingjie; Du, Yong; Iqbal, Muhammad Waleed; Yang, Haoyu; Shen, Xiaolin; Wang, Jia; Sun, Xinxiao & Yuan, Qipeng. Microbial Synthesis of Nucleosides: Advances and Prospects. ACS Synthetic Biology. 2025, 14(1), 1-9.

Liu, Shuang; Kang, Pei; Cui, Zhenzhen; Wang, Zhiwen & Chen, Tao. Increased riboflavin production by knockout of 6-phosphofructokinase I and blocking the Entner–Doudoroff pathway in Escherichia coli. Biotechnology Letters. 2016, 38(8), 1307-1314.