Escherichia coli RF01S/p15Trc-zg
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid p20C-EC10 added | Plasmid p15Trc-zg addedBars (|) indicate differences between strains.
Plasmids
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p20C-EC10 (pBR322 replicon, Ptrc-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)
p15Trc-zg (p15A replication, Ptrc-synRBS-zwf-synRBS-gnd, zwf and gnd genes from C. glutamicum)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Feedback insensitive
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Feedback insensitive
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| riboflavin | Titer | 197.4 mg/L | 3.0 OD600 | Flask | [248] | ||
| riboflavin | Substrate yield | 24.2 mg/g of sustrate | 3.0 OD600 | Flask | [248] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.