Escherichia coli HS

Ancestors

Derived strains

Genotype with respect to parental

ΔJIB* ΔthrB ΔmetA ΔlysA ΔiclR ΔptsG ΔgalR ΔlacI::Ptrc-rhtA Ptrc-rhtA Ptrc-eamA Ptrc-metL Ptrc-thrA Ptrc-glk Ptrc-gltB

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔJIB* ΔthrB ΔmetA ΔlysA ΔiclR ΔptsG ΔgalR ΔlacI::Ptrc-rhtA Ptrc-rhtA Ptrc-eamA Ptrc-metL Ptrc-thrA Ptrc-glk Ptrc-gltB

Bars (|) indicate differences between strains.

Modifications

Origin	Gene	Organism	Modification
Escherichia coli HS	thrB	Escherichia coli	Silencing
Escherichia coli HS	metA	Escherichia coli	Silencing
Escherichia coli HS	lysA	Escherichia coli	Silencing
Escherichia coli HS	iclR	Escherichia coli	Silencing
Escherichia coli HS	ptsG	Escherichia coli	Silencing
Escherichia coli HS	galR	Escherichia coli	Silencing
Escherichia coli HS	lacI	Escherichia coli	Silencing
Escherichia coli HS	rhtA	Escherichia coli	Overexpression
Escherichia coli HS	rhtA	Escherichia coli	Overexpression
Escherichia coli HS	eamA	Escherichia coli	Overexpression
Escherichia coli HS	metL	Escherichia coli	Overexpression
Escherichia coli HS	thrA	Escherichia coli	Overexpression
Escherichia coli HS	glk	Escherichia coli	Overexpression
Escherichia coli HS	gltB	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Scale	Ref.
L-homoserine	Titer	7.31 g/L ^*	11.61 OD₆₀₀^*	Glucose	Flask	[255]
L-homoserine	Titer	13.39 g/L ^*		Glucose	Flask	[255]
L-homoserine	Titer	13.39 g/L ^*	13.29 OD₆₀₀^*	Glucose	Flask	[255]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Niu, Kun; Zheng, Rui; Zhang, Miao; Chen, Mao‐Qin; Kong, Yi‐Ming; Liu, Zhi‐Qiang & Zheng, Yu‐Guo. Adjustment of the main biosynthesis modules to enhance the production of l‐homoserine in Escherichia coli W3110. Biotechnology and Bioengineering. 2024.