Escherichia coli TRTHBB

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli TRTH

Escherichia coli TRTHB

Escherichia coli TRTHBB

Genotype with respect to parental

ΔpoxB

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | trpEDCBA+Tet^R, ΔtnaA | ΔgltB | ΔpoxB

Bars (|) indicate differences between strains.

Modifications

Origin	Gene	Organism	Modification
Escherichia coli TRTH	trpE	Escherichia coli	Overexpression
Escherichia coli TRTH	trpD	Escherichia coli	Overexpression
Escherichia coli TRTH	trpC	Escherichia coli	Overexpression
Escherichia coli TRTH	trpB	Escherichia coli	Overexpression
Escherichia coli TRTH	trpA	Escherichia coli	Overexpression
Escherichia coli TRTH	tnaA	Escherichia coli	Silencing
Escherichia coli TRTHB	gltB	Escherichia coli	Silencing
Escherichia coli TRTHBB	poxB	Escherichia coli	Silencing

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
acetate	Titer	1.41 g/L		Glucose	40 h	Fed-Batch	[159]
L-tryptophan	Substrate yield	16.05 %		Glucose	40 h	Fed-Batch	[159]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Qingyang Xu, Fang Bai, Ning Chen & Gang Bai (2017). Gene modification of the acetate biosynthesis pathway in Escherichia coli and implementation of the cell recycling technology to increase L-tryptophan production. PLOS ONE.