Escherichia coli Y5-3

Ancestors

Derived strains

Genotype with respect to parental

yghe::P_esaS-guaB

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ∆xdhABC | ∆purR | ygay:: P_trc-purF_Bs^{D293V.K316Q.S400W} | yeep::P_trc- prs_Ec^D128A | ilvg::P_trc- purFDNTSQLMKECB_Bs | ycgh:: P_trc- add_Ec, yjiT::P_trc-ade_Ec | ylbe::P_trc-isn1_Sc | ∆hpt | ∆guaB | rph::P_apFAB104-EsaR | yghe::P_esaS-guaB

Bars (|) indicate differences between strains.

Modifications

Origin	Gene	Organism	Modification
Escherichia coli HX1	xdhA	Escherichia coli	Silencing
Escherichia coli HX1	xdhB	Escherichia coli	Silencing
Escherichia coli HX1	xdhC	Escherichia coli	Silencing
Escherichia coli HX2	purR	Escherichia coli	Silencing
Escherichia coli HX3-2	purF	Bacillus subtilis	Feedback insensitive
Escherichia coli HX4-2	prsA	Escherichia coli	Feedback insensitive
Escherichia coli HX5	ilvG	Escherichia coli	Silencing
Escherichia coli HX5	purF	Bacillus subtilis	Overexpression
Escherichia coli HX5	purD	Bacillus subtilis	Overexpression
Escherichia coli HX5	purN	Bacillus subtilis	Overexpression
Escherichia coli HX5	purT	Bacillus subtilis	Overexpression
Escherichia coli HX5	purS	Bacillus subtilis	Overexpression
Escherichia coli HX5	purQ	Bacillus subtilis	Overexpression
Escherichia coli HX5	purL	Bacillus subtilis	Overexpression
Escherichia coli HX5	purM	Bacillus subtilis	Overexpression
Escherichia coli HX5	purK	Bacillus subtilis	Overexpression
Escherichia coli HX5	purE	Bacillus subtilis	Overexpression
Escherichia coli HX5	purC	Bacillus subtilis	Overexpression
Escherichia coli HX5	purB	Bacillus subtilis	Overexpression
Escherichia coli Y3-4	add	Escherichia coli	Overexpression
Escherichia coli Y3-4	adeD	Escherichia coli	Overexpression
Escherichia coli Y4-2	ISN1	Saccharomyces cerevisiae	Overexpression
Escherichia coli Y4-3	hpt	Escherichia coli	Silencing
Escherichia coli Y5-1	guaB	Escherichia coli	Silencing
Escherichia coli Y5-2	rph	Escherichia coli	Silencing
Escherichia coli Y5-2	esaR	Pantoea stewartii	Overexpression
Escherichia coli Y5-3	guaB	Escherichia coli	Attenuation

References

Siyu Zhao, Tangen Shi, Liangwen Li, Zhichao Chen, Changgeng Li, Zichen Yu, Pengjie Sun & Qingyang Xu (2024). The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine. Microbial Cell Factories.