Escherichia coli NMS-19
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔtnaA | ΔqueD | ΔmetJ | Plasmid pAC-DHsI*mlS added | Plasmid pTrc-TBBsEMX addedBars (|) indicate differences between strains.
Plasmids
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pTrc-TBBsEMX (tphDj, phhBPe, folEBs, folM, folX)
pAC-DHsI*mlS (ddcSs, mtnEc, luxSEc, SAM2Sc)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| N-Methylserotonin | Titer | 128.6 mg/L | Fed-Batch | [257] | |||
| N-Methylserotonin | Titer | 5.18 mg/L | 12 h | Flask | [257] | ||
| 5-hydroxy-L-tryptophan | Titer | 252.0 mg/L | 84 h | Fed-Batch | [257] | ||
| 5-hydroxy-L-tryptophan | Titer | 371.5 mg/L | 72 h | Fed-Batch | [257] | ||
| serotonin | Titer | 20.41 mg/L * | Flask | [257] | |||
| 5-hydroxy-L-tryptophan | Titer | 23.53 mg/L * | Flask | [257] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.