Escherichia coli ZH-4
Ancestors
Genotype with respect to parental
Ptac-aroGS211F-Ptac-trpEQ71K/S94N/C465Y-trpABCD-serAH344A/N364A
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | △trpR, △tnaAB | Ptac-aroGS211F-Ptac-trpEQ71K/S94N/C465Y-trpABCD-serAH344A/N364A | Ptac-aroGS211F-Ptac-trpEQ71K/S94N/C465Y-trpABCD-serAH344A/N364A | Ptac-aroGS211F-Ptac-trpEQ71K/S94N/C465Y-trpABCD-serAH344A/N364A | Ptac-aroGS211F-Ptac-trpEQ71K/S94N/C465Y-trpABCD-serAH344A/N364ABars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Feedback insensitive
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Feedback insensitive
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Feedback insensitive
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Feedback insensitive
|
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Overexpression
|
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Overexpression
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Overexpression
|
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Overexpression
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Feedback insensitive
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Feedback insensitive
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Feedback insensitive
|
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|
Overexpression
|
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|
Overexpression
|
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Overexpression
|
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|
Overexpression
|
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|
Feedback insensitive
|
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|
Feedback insensitive
|
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|
Feedback insensitive
|
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|
Overexpression
|
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Overexpression
|
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Overexpression
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Overexpression
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Feedback insensitive
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tryptophan | Titer | 5.33 g/L * | 12.25 OD600* | Flask | [258] | ||
| L-tryptophan | Substrate yield | 0.129 g/g of sustrate * | 12.25 OD600* | Flask | [258] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.