Escherichia coli ispALaFS-NA
Ancestors
Genotype with respect to wild type
F−, endA1, hsdR17 (r K −, m K + ), supE44, thi-l, λ−, recA1, gyrA96, ΔlacU169 (Φ80lacZ ΔM15) | Plasmid pTispALaFS added | Plasmid pSNA addedBars (|) indicate differences between strains.
Plasmids
-
pTispALaFS (Ptrc-RBS-ispA-linker (GGGGS)-aFS)
pSNA (mvaEEf, mvaSEf, mvaK1Sp, mvaK2Sp, mvaDSp and idiEc)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| (E,E)-α-farnesene | Titer | 380.0 mg/L | 21.1 OD600* | 48 h * | Flask | [261] | |
| (R)-mevalonate | Titer | 16.83 mg/L * | 21.1 OD600* | 48 h * | Flask | [261] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.