Escherichia coli LEU23
Ancestors
Derived strains
Genotype with respect to parental
ΔldhA
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | ycjV::Ptrc-leuAfbrEc | yciQ::Ptrc-leuAfbrEc | yghE::Ptrc-leuAfbrEc | yjgX::Ptrc-leuBCD | yjiT::Ptrc-ilvIHfbr | ylbE::Ptrc-ilvIHfbr | yjiV::Ptrc-ilvEDC | PleuA::Ptrc | ΔlivK | livJ::Ptrc-azlC | brnQ::Ptrc-brnFE | yjiP::pntAB | ilvE::Ptrc-Bcldh | ΔpoxB | ΔpflB | ΔldhABars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Feedback insensitive
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Feedback insensitive
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Feedback insensitive
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Overexpression
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Overexpression
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Silencing
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Overexpression
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Overexpression
|
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Silencing
|
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|
Overexpression
|
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Overexpression
|
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|
Silencing
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Overexpression
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Silencing
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Silencing
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Silencing
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-leucine | Titer | 54.6 g/L | 99.5 OD600* | 48 h * | Fed-Batch | [265] | |
| L-leucine | Substrate yield | 0.17 g/g of sustrate | 99.5 OD600* | 48 h * | Fed-Batch | [265] | |
| L-leucine | Titer | 17.15 g/L * | 37.81 OD600* | Flask | [265] | ||
| L-leucine | Substrate yield | 0.14 g/g of sustrate * | 37.81 OD600* | Flask | [265] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.