Escherichia coli HGA5
Strain derived from a randomly mutated strain.
Ancestors
Genotype with respect to parental
ΔtyrR, pheA and adhE
Genotype with respect to wild type
F+ (λ) | l-Tyrosine auxotrophic | ΔtyrR, pheA and adhE | Plasmid pAP12 addedBars (|) indicate differences between strains.
Plasmids
-
pAP12 (pAP with aroGfbr, tyrAfbr and aroABC, p15A ori with PRPL)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Silencing
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Feedback insensitive
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tyrosine | Titer | 3.2 g/L * | 48 h * | Flask | [268] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.