Escherichia coli FB-03/pSV03
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔtnaA | ΔpheA | Plasmid pSV03 addedBars (|) indicate differences between strains.
Plasmids
-
pSV03 (p15A ori, aroFfbr trpEfbrD)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Silencing
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Feedback insensitive
|
|||
|
Feedback insensitive
|
|||
|
Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tryptophan | Titer | 10.8 g/L | 40.8 g/L | Fed-Batch | [147] | ||
| L-tryptophan | Substrate yield | 0.07 g/g of sustrate | 40.8 g/L | Fed-Batch | [147] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.