Escherichia coli FB-04/pSV03

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔtnaA | ΔpheA | ΔtyrA::FRT | Plasmid pSV03 added

Bars (|) indicate differences between strains.


Plasmids

  • pSV03 (p15A ori, aroFfbr trpEfbrD)


Modifications
Origin Gene Organism Modification
Silencing
Overexpression
Overexpression
Overexpression
Overexpression
Overexpression
Silencing
Silencing
Silencing
Feedback insensitive
Feedback insensitive
Overexpression

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 13.3 g/L 41.2 g/L Fed-Batch [147]
L-tryptophan Substrate yield 0.1 g/g of sustrate 41.2 g/L Fed-Batch [147]
L-tryptophan Titer 1.1 g/L 7.0 OD600 48 h Flask [146]
L-tryptophan Substrate yield 0.02 g/g of sustrate 7.0 OD600 48 h Flask [146]
L-tryptophan Volumetric productivity 0.02 g/L/h 7.0 OD600 48 h Flask [146]
acetate Titer 12.9 g/L 7.0 OD600 48 h Flask [146]
L-tryptophan Titer 38.1 g/L 73.6 OD600 54 h Fed-Batch [146]
L-tryptophan Substrate yield 0.11 g/g of sustrate 73.6 OD600 54 h Fed-Batch [146]
L-tryptophan Volumetric productivity 0.7 g/L/h 73.6 OD600 54 h Fed-Batch [146]
acetate Titer 4.3 g/L 73.6 OD600 54 h Fed-Batch [146]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References