Escherichia coli HM4 (pBRmetL–pNrhtA)

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | ΔlysA | ΔmetA | ΔthrBC | Plasmid pBRmetL–pNrhtA added

Bars (|) indicate differences between strains.


Plasmids

Modifications
Origin Gene Organism Modification
Silencing
Silencing
Silencing
Silencing
Silencing
Overexpression
Overexpression

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-homoserine Titer 2.12 g/L 44 h Flask [272]
L-homoserine Titer 33.42 g/L 40 h Flask [272]
L-homoserine Substrate yield 0.26 g/g of sustrate 40 h Flask [272]
L-homoserine Titer 33.42 g/L 13.36 g/L 44 h Fed-Batch [272]
L-homoserine Substrate yield 0.24 g/g of sustrate 13.36 g/L 44 h Fed-Batch [272]
acetate Titer 3.54 g/L 13.36 g/L 44 h Fed-Batch [272]
acetate Substrate yield 0.027 g/g of sustrate 13.36 g/L 44 h Fed-Batch [272]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References