Escherichia coli HTP101-LMT
Ancestors
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | ΔtnaA | Plasmid pSC101trp added | Plasmid pACHTP-LMT addedBars (|) indicate differences between strains.
Plasmids
-
pACHTP-LMT (p15A ori, Lacpro-mtrA-PTPS-SPR, M1-93pro-PCD-DHPR, T7pro-TPH145)
pSC101trp (pSC101 ori, tacpro-aroHfbr, tacpro-trpEfbrDCBA)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Feedback insensitive
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tryptophan | Titer | 1.54 g/L | 15.97 OD600 | 90 h | Flask | [273] | |
| 5-hydroxy-L-tryptophan | Titer | 1.21 g/L | 15.97 OD600 | 90 h | Flask | [273] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.