Escherichia coli S028/pTAC242
In this strain, the impact of the empty vector is evaluated.
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to parental
pTac
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacU169 gal490 λcI857 Δ(cro-bioA) | rpsL (StrR) | ∆aroF ∆aroG ∆mtr ∆tnaA ∆tnaB ∆λ | ∆aroH::PJ23119-rpsL-tac -(aroGfbr-serAfbr) | Ptrc -trpEfbrDCBA | pTac | Plasmid pTAC242 addedBars (|) indicate differences between strains.
Plasmids
-
pTAC242 (pSC101 ori, Ptac)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Feedback insensitive
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Feedback insensitive
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tryptophan | Substrate yield | 0.14 g/g of sustrate | 42 h | Fed-Batch | [165] | ||
| L-tryptophan | Titer | 19.0 g/L | 42 h | Fed-Batch | [165] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.