Escherichia coli EIP81

Ancestors

Derived strains

• Escherichia coli ENP
• Escherichia coli ENO1
• Escherichia coli ENB1
• Escherichia coli ENB2
• Escherichia coli ENB3
• Escherichia coli ENB14
• Escherichia coli ENB27
• Escherichia coli ENB33
• Escherichia coli ENB36
• Escherichia coli ENB41
• Escherichia coli ENB48
• Escherichia coli ENB49
• Escherichia coli ENB50
• Escherichia coli ENB51
• Escherichia coli ENB52
• Escherichia coli ENB53
• Escherichia coli ENB54
• Escherichia coli ENB55
• Escherichia coli ENB56
• Escherichia coli ENB57
• Escherichia coli ENB58
• Escherichia coli ENO2

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | (DE3) | Plasmid pISP5 added | Plasmid pMAP6 added

Bars (|) indicate differences between strains.

Plasmids

• pMAP6 (pBR322 origin, P_T7-EfmvaE, P_T7-EfmvaS^A110G)

  pISP5 (P_T7-MmMK-ScPMK-ScPMD-ScIDI)

References

• Dengwei Lei, Zetian Qiu, Jihua Wu, Bin Qiao, Jianjun Qiao & Guang-Rong Zhao (2021). Combining Metabolic and Monoterpene Synthase Engineering for de Novo Production of Monoterpene Alcohols in Escherichia coli. ACS Synthetic Biology.