Escherichia coli MGΔpgi

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli MGΔpgi

Genotype with respect to parental

Δpgi

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Δpgi | Plasmid pTrc99a-iaa9 added | Plasmid p6LRPW326 added

Bars (|) indicate differences between strains.

Plasmids

p6LRPW326 (trp operon, aroG^fbr, trpE^fbr, and serA^fbr)

pTrc99a-iaa9 (P_trc-iaaM-P_tac-ami1-P_tac-ami1)

Modifications

Origin	Gene	Organism	Modification
Escherichia coli MGΔpgi	pgi	Escherichia coli	Silencing
p6LRPW326	trpE	Escherichia coli	Overexpression
p6LRPW326	trpD	Escherichia coli	Overexpression
p6LRPW326	trpC	Escherichia coli	Overexpression
p6LRPW326	trpB	Escherichia coli	Overexpression
p6LRPW326	trpA	Escherichia coli	Overexpression
p6LRPW326	aroG	Escherichia coli	Feedback insensitive
p6LRPW326	trpE	Escherichia coli	Feedback insensitive
p6LRPW326	serA	Escherichia coli	Feedback insensitive
pTrc99a-iaa9	iaaM	Pseudomonas savastanoi	Overexpression
pTrc99a-iaa9	ami1	Arabidopsis thaliana	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan	Titer	1.015 g/L		Glucose	48 h	Flask	[289]
(indol-3-yl)acetate	Titer	0.8 g/L ^*		Glucose	96 h ^*	Flask	[289]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Wu, Hongxuan; Yang, Jinhua; Shen, Peijie; Li, Qingchen; Wu, Weibin; Jiang, Xianzhang; Qin, Lina; Huang, Jianzhong; Cao, Xiao & Qi, Feng. High-Level Production of Indole-3-acetic Acid in the Metabolically Engineered Escherichia coli. Journal of Agricultural and Food Chemistry. 2021, 69(6), 1916-1924.