Escherichia coli MG1655-trp

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pTrc99a-iaa9 added | Plasmid p6LRPW326 added

Bars (|) indicate differences between strains.

p6LRPW326 (trp operon, aroG^fbr, trpE^fbr, and serA^fbr)

pTrc99a-iaa9 (P_trc-iaaM-P_tac-ami1-P_tac-ami1)

Origin	Gene	Organism	Modification
p6LRPW326	trpE	Escherichia coli	Overexpression
p6LRPW326	trpD	Escherichia coli	Overexpression
p6LRPW326	trpC	Escherichia coli	Overexpression
p6LRPW326	trpB	Escherichia coli	Overexpression
p6LRPW326	trpA	Escherichia coli	Overexpression
p6LRPW326	aroG	Escherichia coli	Feedback insensitive
p6LRPW326	trpE	Escherichia coli	Feedback insensitive
p6LRPW326	serA	Escherichia coli	Feedback insensitive
pTrc99a-iaa9	iaaM	Pseudomonas savastanoi	Overexpression
pTrc99a-iaa9	ami1	Arabidopsis thaliana	Overexpression

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
(indol-3-yl)acetate	Titer	0.7 g/L ^*		Glucose	96 h ^*	Flask	[289]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.