Escherichia coli Arg6

Strain derived from a randomly mutated strain.

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli Arg0

Escherichia coli Arg1

Escherichia coli Arg2

Escherichia coli Arg3

Escherichia coli Arg4

Escherichia coli Arg5

Escherichia coli Arg6

Derived strains

Genotype with respect to parental

P_icd:P_BJ23100-B0034

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | argA^K204T, argR^Y33X, pyrG^D450H, and speB^E189A | △speC △speF | argE^Ec::P_BJ23119-B0034-argJ^Tn | yghX::P_BJ23119-B0034-argCBH^Ec, yncI::P_BJ23119-B0034-argA*, rpnD::P_BJ23119-B0034-argD, ycjV::P_BJ23119-B0034-argI and yrfG::P_BJ23119-B0034-argG | artJ::P_BJ23119-B0034-lysE^Cg and argT::P_BJ23119-B0034-cgmA^Cg | yacC::P_BJ23119-B0034-gdhA^St and ydjK::P_BJ23119-B0034-gdhA^St | P_icd:P_BJ23100-B0034

Bars (|) indicate differences between strains.

Modifications

Origin	Gene	Organism	Modification
Escherichia coli Arg0	argA	Escherichia coli	Feedback insensitive
Escherichia coli Arg0	argA	Escherichia coli	Feedback insensitive
Escherichia coli Arg0	argR	Escherichia coli	Silencing
Escherichia coli Arg0	pyrG	Escherichia coli	Unstated
Escherichia coli Arg0	speB	Escherichia coli	Attenuation
Escherichia coli Arg1	speC	Escherichia coli	Silencing
Escherichia coli Arg1	speF	Escherichia coli	Silencing
Escherichia coli Arg2	argE	Escherichia coli	Silencing
Escherichia coli Arg2	argJ	Thermotoga neapolitana	Overexpression
Escherichia coli Arg3	argD	Escherichia coli	Overexpression
Escherichia coli Arg3	argI	Escherichia coli	Overexpression
Escherichia coli Arg3	argG	Escherichia coli	Overexpression
Escherichia coli Arg3	yrfG	Escherichia coli	Silencing
Escherichia coli Arg3	ycjV	Escherichia coli	Silencing
Escherichia coli Arg3	yghX	Escherichia coli	Silencing
Escherichia coli Arg3	argC	Escherichia coli	Overexpression
Escherichia coli Arg3	argB	Escherichia coli	Overexpression
Escherichia coli Arg3	argH	Escherichia coli	Overexpression
Escherichia coli Arg3	yncI	Escherichia coli	Silencing
Escherichia coli Arg3	argA	Escherichia coli	Feedback insensitive
Escherichia coli Arg3	rpnD	Escherichia coli	Silencing
Escherichia coli Arg4	artJ	Escherichia coli	Silencing
Escherichia coli Arg4	lysE	Corynebacterium glutamicum	Overexpression
Escherichia coli Arg4	argT	Escherichia coli	Silencing
Escherichia coli Arg4	lysG	Corynebacterium glutamicum	Overexpression
Escherichia coli Arg5	yacC	Escherichia coli	Silencing
Escherichia coli Arg5	gdhA	Salmonella typhimurium	Overexpression
Escherichia coli Arg5	ydjK	Escherichia coli	Silencing
Escherichia coli Arg6	icd	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Carbon source	Scale	Ref.
L-arginine	Titer	23.08 g/L	Glucose	Flask	[301]
L-arginine	Titer	71.27 g/L	Glucose	Fed-Batch	[301]
L-arginine	Substrate yield	0.38 g/g of sustrate	Glucose	Fed-Batch	[301]
L-arginine	Volumetric productivity	1.48 g/L/h	Glucose	Fed-Batch	[301]
L-arginine	Titer	4.75 g/L	Glucose	Fed-Batch	[301]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Sheng, Qi; He, Shengyang; Liang, Guangjie; Meng, Gang; Zhao, Chunguang; Wei, Aiying; Gou, Lining; Liu, Jia; Li, Xiaomin; Wu, Jing & Liu, Liming. Improved arginine production in Escherichia coli by harnessing the intracellular citrulline. Metabolic Engineering. 2025.