Escherichia coli Arg8
Strain derived from a randomly mutated strain.
Synonyms
Arg7-3
Ancestors
Derived strains
Genotype with respect to parental
PBJ23110-B0034-aspC and PBJ23115-B0034-gltA
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | argAK204T, argRY33X, pyrGD450H, and speBE189A | △speC △speF | argEEc::PBJ23119-B0034-argJTn | yghX::PBJ23119-B0034-argCBHEc, yncI::PBJ23119-B0034-argA*, rpnD::PBJ23119-B0034-argD, ycjV::PBJ23119-B0034-argI and yrfG::PBJ23119-B0034-argG | artJ::PBJ23119-B0034-lysECg and argT::PBJ23119-B0034-cgmACg | yacC::PBJ23119-B0034-gdhASt and ydjK::PBJ23119-B0034-gdhASt | Picd:PBJ23100-B0034 | Pppc:PBJ23114-B0034 | PBJ23110-B0034-aspC and PBJ23115-B0034-gltABars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Feedback insensitive
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Feedback insensitive
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Silencing
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Unstated
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Attenuation
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Feedback insensitive
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Silencing
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Silencing
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Overexpression
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Silencing
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Overexpression
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Silencing
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Overexpression
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Attenuation
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-arginine | Titer | 30.01 g/L | Flask | [301] | |||
| L-arginine | Titer | 80.51 g/L | Fed-Batch | [301] | |||
| L-arginine | Substrate yield | 0.42 g/g of sustrate | Fed-Batch | [301] | |||
| L-arginine | Volumetric productivity | 1.68 g/L/h | Fed-Batch | [301] | |||
| L-citrulline | Titer | 2.33 g/L | Fed-Batch | [301] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.