Escherichia coli Arg4-6
Strain derived from a randomly mutated strain.
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | argAK204T, argRY33X, pyrGD450H, and speBE189A | △speC △speF | argEEc::PBJ23119-B0034-argJTn | yghX::PBJ23119-B0034-argCBHEc, yncI::PBJ23119-B0034-argA*, rpnD::PBJ23119-B0034-argD, ycjV::PBJ23119-B0034-argI and yrfG::PBJ23119-B0034-argG | artJ::PBJ23119-B0034-lysECg and argT::PBJ23119-B0034-cgmACg | Plasmid pCDR-PBJ23119-B0034-argF addedBars (|) indicate differences between strains.
Plasmids
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pCDR-PBJ23119-B0034-argF (CloDF13 ori, PBJ23119-B0034-argF)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Feedback insensitive
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Feedback insensitive
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Silencing
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Unstated
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Attenuation
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Feedback insensitive
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Silencing
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Silencing
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Overexpression
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Silencing
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-arginine | Titer | 15.31 g/L | Flask | [301] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.