Escherichia coli KW005
Silencing pykA and pykF decreases PEP consumption but simultaneously limits ATP generation, thereby impairing cellular growth and diminishing tryptophan production.
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Strain derived from a randomly mutated strain.
Ancestors
Genotype with respect to parental
ΔpykF ΔpykA
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid PH5a1 added | ΔtrpR ΔtrpL | ΔpykF ΔpykABars (|) indicate differences between strains.
Plasmids
-
PH5a1 (aroGfbr trpEDCBA)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Silencing
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tryptophan | Titer | 0.1 g/L | Flask | [135] | |||
| L-tryptophan | Substrate yield | 0.1 g/g of sustrate | Flask | [135] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.