Escherichia coli BL21(DE3)/pET24a-Asn-Ec

Ancestors

Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | Plasmid pET24a/AsnA-Ec added

Bars (|) indicate differences between strains.


Plasmids

Modifications
Origin Gene Organism Modification
Overexpression
Overexpression

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-asparagine Titer 2.7 g/L 36 h Flask [309]
L-asparagine Titer 38.49 g/L 20 h Fed-Batch [309]
L-asparagine Substrate yield 0.2916 mol/mol of substrate 20 h Fed-Batch [309]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References