Escherichia coli BW15-3/pED
Ancestors
Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | ∆tnaA | ∆yhaM | yijP::PTrc-serAf | ydeU::PTrc-serC | mbhA::PTrc-serB | ∆sdaA | ∆sdaB | ∆tdcG | PglyA(-125T-126C)-glyA | yeeP::PTrc-cysM | gapC::PTrc-nrdH | yafF::PTrc-glpE | ycdN::PTrc-fbaA | lfhA::PTrc-talA | ycgH::PTrc-gapNSm | Plasmid pTrc99a-cysEf-ydeD addedBars (|) indicate differences between strains.
Plasmids
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pTrc99a-cysEf-ydeD (Ptrc-cysEf-ydeD)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Silencing
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Feedback insensitive
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Overexpression
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Silencing
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Overexpression
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Silencing
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Silencing
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Silencing
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Attenuation
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Silencing
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Overexpression
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Silencing
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Overexpression
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Silencing
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Overexpression
|
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Silencing
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|
Overexpression
|
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Silencing
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|
Overexpression
|
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Silencing
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Overexpression
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Feedback insensitive
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-cysteine | Titer | 12.6 g/L | 47.9 OD600 | Fed-Batch | [310] | ||
| L-cysteine | Substrate yield | 0.08 g/g of sustrate | 47.9 OD600 | Fed-Batch | [310] | ||
| L-cysteine | Volumetric productivity | 0.21 g/L/h | 47.9 OD600 | Fed-Batch | [310] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.
References
- Yang, Hui; Zhang, Bo; Wu, Zi-Dan; Chen, Li-Feng; Pan, Jia-Yuan; Xiu, Xiao-Ling; Cai, Xue; Liu, Zhi-Qiang & Zheng, Yu-Guo. Combinatorial Metabolic Engineering of Escherichia coli for Enhanced L-Cysteine Production: Insights into Crucial Regulatory Modes and Optimization of Carbon-Sulfur Metabolism and Cofactor Availability. Journal of Agricultural and Food Chemistry. 2023, 71(36), 13409-13418.