Escherichia coli MG1655/pBluescript II SK(+)/pSTV29/pLS19
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pBluescriptII KS(+) added | Plasmid pLS219 added | Plasmid pSTV29 addedBars (|) indicate differences between strains.
Plasmids
-
pSTV29 (p15a ori)
pBluescriptII KS(+) (pUC ori, f1 ori, Plac-lacZ')
pLS219 ()
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-cysteine | Titer | 0.0 mg/L * | 24 h * | Flask | [318] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.