Escherichia coli ΔcysM/pYceA

Ancestors

All strains

Escherichia coli K12

Escherichia coli BW25113

Escherichia coli JW2414

Escherichia coli ΔcysM/pYceA

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | cysM::Km^R | Plasmid pYceA added

Bars (|) indicate differences between strains.

Plasmids

pYceA (P_T5-lac-6xHis-yceA)

Modifications

Origin	Gene	Organism	Modification
Escherichia coli JW2414	cysM	Escherichia coli	Silencing
pYceA	trhO	Escherichia coli	Overexpression

References

Kawano, Yusuke; Onishi, Fumito; Shiroyama, Maeka; Miura, Masashi; Tanaka, Naoyuki; Oshiro, Satoshi; Nonaka, Gen; Nakanishi, Tsuyoshi & Ohtsu, Iwao. Improved fermentative l-cysteine overproduction by enhancing a newly identified thiosulfate assimilation pathway in Escherichia coli. Applied Microbiology and Biotechnology. 2017, 101(18), 6879-6889.