Escherichia coli EF-4-2
Ancestors
Genotype with respect to parental
ribF::ribCG199D
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | △lacI | Plasmid pduet-rib added | △pfkA | △edd△eda | △ribB-FMN riboswitch | ribF::ribCG199DBars (|) indicate differences between strains.
Plasmids
-
pduet-rib (Ptac-ribEDAH)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Silencing
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Silencing
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Feedback insensitive
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Silencing
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Attenuation
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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References
- Zhang, Xiaoling; Li, Yanan; Wang, Kang; Yin, Jilong; Du, Yuxuan; Yang, Zhen; Pan, Xuewei; You, Jiajia & Rao, Zhiming. Construction of antibiotic-free riboflavin producer in Escherichia coli by metabolic engineering strategies with a plasmid stabilization system. Synthetic and Systems Biotechnology. 2025, 10(2), 346-355.