Escherichia coli CWF4NA/pTac15kPTA/pSynPPC1
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔiclR ΔfumC ΔfumA ΔfumB ΔptsG ΔlacI | PaspA::Ptrc | Plasmid pSynPPC1 added | Plasmid pTac15kPTA addedBars (|) indicate differences between strains.
Plasmids
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pTac15kPTA (Ptac-RBS(ptrc)-panD-aspA)
pSynPPC1 (PJ23100-RBSppc-ppc)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| β-alanine | Titer | 1.66 g/L | Flask | [330] | |||
| fumarate | Titer | 2.59 g/L | Flask | [330] | |||
| β-alanine | Titer | 3.49 g/L | Flask | [330] | |||
| fumarate | Titer | 1.0 g/L | Flask | [330] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.