Escherichia coli AC03

Strain derived from a randomly mutated strain.

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli AC

Escherichia coli AC02

Escherichia coli AC03

Derived strains

Escherichia coli AC04

Genotype with respect to parental

△gabD

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | High lysine-producing strain | Plasmid pLADH added | Plasmid pLADH lost | Plasmid pADCTKC added | Plasmid pBD added | △gabD

Bars (|) indicate differences between strains.

Plasmids

pBD (car_{_Mm}-sfp-yqhD)

pADCTKC (p15A ori, P_trc, patAD-cadA-gabT-yahK-cgl0841)

Modifications

Origin	Gene	Organism	Modification
Escherichia coli AC03	gabD	Escherichia coli	Silencing
pBD	fadD9	Mycobacterium marinum	Overexpression
pBD	sfp	Bacillus subtilis	Overexpression
pBD	yqhD	Escherichia coli	Overexpression
pADCTKC	patA	Escherichia coli	Overexpression
pADCTKC	patD	Escherichia coli	Overexpression
pADCTKC	cadA	Escherichia coli	Overexpression
pADCTKC	gabT	Escherichia coli	Overexpression
pADCTKC	yahK	Escherichia coli	Overexpression
pADCTKC	cgl0841	Corynebacterium glutamicum	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
1,5-pentanediol	Titer	1.88 g/L		Glucose	60 h	Flask	[331]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Cen, Xuecong; Liu, Yanjuan; Zhu, Fanghuan; Liu, Dehua & Chen, Zhen. Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway. Metabolic Engineering. 2022, 74, 168-177.