Escherichia coli AC04

Strain derived from a randomly mutated strain.

Ancestors

Derived strains

Genotype with respect to parental

△gdhA


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | High lysine-producing strain | Plasmid pLADH added | Plasmid pLADH lost | Plasmid pADCTKC added | Plasmid pBD added | △gabD | △gdhA

Bars (|) indicate differences between strains.


Plasmids

  • pBD (car_Mm-sfp-yqhD)

    pADCTKC (p15A ori, Ptrc, patAD-cadA-gabT-yahK-cgl0841)


Modifications
Origin Gene Organism Modification
Silencing
Silencing
pBD
Overexpression
pBD
sfp
Overexpression
pBD
Overexpression
Overexpression
Overexpression
Overexpression
Overexpression
Overexpression
Overexpression

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
1,5-pentanediol Titer 2.62 g/L Flask [331]
cadaverine Titer 1.56 g/L Flask [331]
1,5-pentanediol Titer 9.25 g/L 172 g Fed-Batch [331]
1,5-pentanediol Substrate yield 0.16 g/g of sustrate 172 h Fed-Batch [331]
1,5-pentanediol Substrate yield 0.28 mol/mol of substrate 172 h Fed-Batch [331]
cadaverine Titer 2.23 g/L 74 h Fed-Batch [331]
cadaverine Titer 0.44 g/L 172 h Fed-Batch [331]
acetate Titer 5.3 g/L 172 h Fed-Batch [331]
5-hydroxypentanoate Titer 3.72 g/L 172 h Fed-Batch [331]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References