Escherichia coli TrpD-Pl/pACJ23-CtAAAH-F
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacU169 gal490 λcI857 Δ(cro-bioA) | rpsL (StrR) | ∆aroF ∆aroG ∆mtr ∆tnaA ∆tnaB ∆λ | ∆aroH::PJ23119-rpsL-tac -(aroGfbr-serAfbr) | Ptrc -trpEfbrDCBA | ∆TrpR | Plasmid pBbE1k-2.2 added | Plasmid pACPJ23-CtAAAH-F addedBars (|) indicate differences between strains.
Plasmids
-
pBbE1k-2.2 (PCDHs and DHPRHs)
pACPJ23-CtAAAH-F (CtAAAHW192F)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Feedback insensitive
|
|||
|
Feedback insensitive
|
|||
|
Feedback insensitive
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Silencing
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| 5-hydroxy-L-tryptophan | Titer | 100.4 mg/L | 48 h | Flask | [338] | ||
| L-tryptophan | Titer | 2.7 g/L | Flask | [338] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.