Escherichia coli HIS1-5
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to parental
yghX::Ptrc-hisDCBHAFI
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI, ylbE::Ptrc-CghisGfbr | hisLG::Ptrc | ilvG::Ptrc-CghisGfbr | ycgH::Ptrc-CghisGfbr | yghX::Ptrc-hisDCBHAFIBars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Feedback insensitive
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
|
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Silencing
|
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Feedback insensitive
|
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|
Silencing
|
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|
Feedback insensitive
|
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|
Silencing
|
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|
Overexpression
|
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Overexpression
|
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Overexpression
|
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Overexpression
|
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-histidine | Titer | 0.36 g/L | 12.6 OD600 | Flask | [340] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.