Escherichia coli HIS3-12
Ancestors
Derived strains
Genotype with respect to parental
mbhA::Ptrc-Ecndk
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI, ylbE::Ptrc-CghisGfbr | hisLG::Ptrc | ilvG::Ptrc-CghisGfbr | ycgH::Ptrc-CghisGfbr | ygaY::Ptrc-CghisGfbr | tfaD::Ptrc-CghisGfbr | ycdN::Ptrc-CghisGfbr | yjiT::Ptrc-EcargO | purR::Ptrc-Smprs | ΔmazG, Δamn, ΔygdH, ΔyrfG, ΔyjjG, ΔsurE, ΔushA | yncI::Ptrc-cipB-hisHAFI, rph::Ptrc-cipB-EcpurH | yghX::Ptrc-hisDCB | purR::Ptrc-cipA-Smprs, yjhE::Ptrc-cipA-tktA | yghE::Ptrc-cipA-Smprs | yeeP::Ptrc-zwf | ybeM::Ptrc-gnd | yjiP::Ptrc-BspurA | yjgX::Ptrc-BspurB | mbhA::Ptrc-EcndkBars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Silencing
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Silencing
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Feedback insensitive
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Silencing
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Silencing
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Feedback insensitive
|
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Silencing
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Feedback insensitive
|
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Silencing
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Feedback insensitive
|
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Silencing
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Feedback insensitive
|
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Silencing
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Feedback insensitive
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Silencing
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Overexpression
|
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Silencing
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Overexpression
|
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Silencing
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Overexpression
|
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Overexpression
|
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Overexpression
|
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Overexpression
|
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Overexpression
|
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|
Silencing
|
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Overexpression
|
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Overexpression
|
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|
Silencing
|
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|
Overexpression
|
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Overexpression
|
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|
Overexpression
|
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|
Silencing
|
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|
Overexpression
|
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|
Overexpression
|
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|
Silencing
|
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|
Overexpression
|
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|
Overexpression
|
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|
Silencing
|
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|
Overexpression
|
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|
Overexpression
|
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|
Silencing
|
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|
Overexpression
|
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|
Silencing
|
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|
Overexpression
|
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|
Silencing
|
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|
Overexpression
|
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|
Silencing
|
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|
Overexpression
|
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Silencing
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-histidine | Titer | 7.28 g/L | Flask | [340] | |||
| L-histidine | Titer | 40.6 g/L | 36 h | Fed-Batch | [340] | ||
| L-histidine | Substrate yield | 0.18 g/g of sustrate | 36 h | Fed-Batch | [340] | ||
| L-histidine | Volumetric productivity | 1.13 g/L/h | 36 h | Fed-Batch | [340] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.