Escherichia coli T14
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to parental
ΔpheA ΔtyrA
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI, tyrR::Ptrc-aroGS180F, trpR::Ptrc-serAH344A,N364A, trpE::Ptrc-trpES40F, ΔpykF, tktA::Ptrc-tktA, ΔptsH, yjiV::PJ23105-UTRWP1-galP, ylbE::PJ23114-UTRMK3-glk, PrpsT p1-ppc, Prrnc p1-gltA | ycjv::Ptrc-aroEK | mbhA::Ptrc-BS.glnAL159I,E304A | yeeP::PserB-serB, PserC-serC | ΔTnaB | ΔpheA ΔtyrABars (|) indicate differences between strains.
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Silencing
|
|||
|
Silencing
|
|||
|
Feedback insensitive
|
|||
|
Silencing
|
|||
|
Feedback insensitive
|
|||
|
Feedback insensitive
|
|||
|
Silencing
|
|||
|
Overexpression
|
|||
|
Silencing
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Feedback insensitive
|
|||
|
Overexpression
|
|||
|
Overexpression
|
|||
|
Silencing
|
|||
|
Silencing
|
|||
|
Silencing
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tryptophan | Titer | 12.29 g/L | 6.68 OD600* | Flask | [183] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.